Intracellular free iron and its potential role in ultrahigh-pressure-induced inactivation of Escherichia coli

Appl Environ Microbiol. 2013 Jan;79(2):722-4. doi: 10.1128/AEM.02202-12. Epub 2012 Nov 2.


Intracellular free iron of Escherichia coli was determined by whole-cell electron paramagnetic resonance spectrometry. Ultrahigh pressure (UHP) increased both intracellular free iron and cell lethality in a pressure-dose-dependent manner. The iron chelator 2,2'-dipyridyl protected cells against UHP treatments. A mutation that produced iron overload conditions sensitized E. coli to UHP treatment.

MeSH terms

  • Electron Spin Resonance Spectroscopy
  • Escherichia coli / chemistry
  • Escherichia coli / physiology*
  • Hydrostatic Pressure*
  • Iron / analysis
  • Iron / toxicity*
  • Microbial Viability / drug effects*


  • Iron