Susceptibility test of two Ca(2+)-ATPase conformers to denaturants and polyols to outline their structural difference

J Membr Biol. 2013 Feb;246(2):141-9. doi: 10.1007/s00232-012-9513-8. Epub 2012 Nov 3.

Abstract

To determine the effect of denaturants [guanidine hydrochloride (GdnHCl) and urea] and polyols [with various molecular masses (62.1-600)] on calcium binding at the two hypothesized conformers (A and B forms) of the chemically equivalent sarcoplasmic reticulum Ca(2+)-ATPase, which bind two calcium ions in different manners, we examined the effect of these reagents on the calcium dependence of ATP-supported phosphorylation of the ATPase molecules and of their calcium-activated, acetyl phosphatate hydrolytic activity. (1) GdnHCl (~0.05 M) and urea (~0.5 M) increased the apparent calcium affinity (K (0.5)) of 2-6 μM of noncooperative binding [Hill coefficient (n (H)) ~ 1] of the A form to 10-40 μM. (2) The employed polyols transformed the binding of the A form into cooperative binding (n (H) ~ 2), accompanying the approach of its K (0.5) value to that (K (0.5) = 0.04-0.2 μM) of the cooperative binding (n (H) ~ 2) of the B form; the transition concentration (0.025-2 M) of the polyols, above which such transformation occurs, was in inverse relation to their molecular mass. (3) The binding of the B form was resistant to these denaturants and polyols. Based on these data, a structural model of the two forms, calcium-binding domains of which are loosely and compactly folded, is presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Guanidine / pharmacology
  • Models, Biological
  • Phosphorylation / drug effects
  • Polymers / pharmacology
  • Rabbits
  • Sarcoplasmic Reticulum / drug effects
  • Sarcoplasmic Reticulum / metabolism
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism*

Substances

  • Polymers
  • polyol
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Guanidine