Ion mobility-mass spectrometry analysis of serum N-linked glycans from esophageal adenocarcinoma phenotypes

J Proteome Res. 2012 Dec 7;11(12):6102-10. doi: 10.1021/pr300756e. Epub 2012 Nov 5.

Abstract

Three disease phenotypes, Barrett's esophagus (BE), high-grade dysplasia (HGD), esophageal adenocarcinoma (EAC), and a set of normal control (NC) serum samples are examined using a combination of ion mobility spectrometry (IMS), mass spectrometry (MS), and principal component analysis (PCA) techniques. Samples from a total of 136 individuals were examined, including 7 characterized as BE, 12 as HGD, 56 as EAC, and 61 as NC. In typical data sets, it was possible to assign ∼20 to 30 glycan ions based on MS measurements. Ion mobility distributions for these ions show multiple features. In some cases, such as the [S1H5N4+3Na]3+ and [S1F1H5N4+3Na]3+ glycan ions, the ratio of intensities of high-mobility features to low-mobility features vary significantly for different groups. The degree to which such variations in mobility profiles can be used to distinguish phenotypes is evaluated for 11 N-linked glycan ions. An outlier analysis on each sample class followed by an unsupervised PCA using a genetic algorithm for pattern recognition reveals that EAC samples are separated from NC samples based on 46 features originating from the 11-glycan composite IMS distribution.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Aged
  • Aged, 80 and over
  • Algorithms
  • Barrett Esophagus / metabolism
  • Barrett Esophagus / pathology
  • Case-Control Studies
  • Computational Biology / methods
  • Early Detection of Cancer / methods
  • Esophageal Neoplasms / metabolism*
  • Esophageal Neoplasms / pathology
  • Esophagus / pathology*
  • Female
  • Humans
  • Ions / metabolism
  • Male
  • Middle Aged
  • Pattern Recognition, Automated
  • Phenotype*
  • Polysaccharides / blood*
  • Principal Component Analysis
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Ions
  • Polysaccharides

Supplementary concepts

  • Adenocarcinoma Of Esophagus