Physical properties and in vitro evaluation of collagen-chitosan-calcium phosphate microparticle-based scaffolds for bone tissue regeneration

J Biomater Appl. 2013 Nov;28(4):566-79. doi: 10.1177/0885328212465662. Epub 2012 Nov 5.


Due to limitations of bone autografts and allografts, synthetic bone grafts using osteoconductive biomaterials have been designed. In this study, collagen-chitosan-calcium phosphate microparticle-based scaffolds fused with glycolic acid were compared to their counterparts without collagen in terms of degradation, cytocompatibility, porosity, and Young's modulus. It was found that 26-30% collagen was incorporated and that hydroxyapatite was present. Moreover, there were no differences between control and collagen scaffolds in degradation, cytocompatibility, porosity, and Young's modulus. In general, scaffolds exhibited 23% porosity, 0.6-1.2 MPa Young's modulus, 23% degradation over 4 weeks, and supported a four to seven fold increase in osteoblast cell number over 7 days in culture. Collagen can be incorporated into these bone graft substitute scaffolds, which show an improved degradation profile.

Keywords: Chitosan; Young’s modulus; calcium phosphate; degradation; synthetic bone graft.

MeSH terms

  • Bone Development*
  • Calcium Phosphates / chemistry*
  • Chitosan / chemistry*
  • Collagen / chemistry*
  • In Vitro Techniques
  • Regeneration*


  • Calcium Phosphates
  • Collagen
  • Chitosan
  • calcium phosphate