Scarecrow Plays a Role in Establishing Kranz Anatomy in Maize Leaves

Plant Cell Physiol. 2012 Dec;53(12):2030-7. doi: 10.1093/pcp/pcs147. Epub 2012 Nov 4.

Abstract

More than a quarter of the primary productivity on land, and a large fraction of the food that humans consume, is contributed by plants that fix atmospheric CO(2) by C(4) photosynthesis. It has been estimated that transferring the C(4) pathway to C(3) crops could boost yield by 50% and also increase water use efficiency and reduce the need for fertilizer, particularly in dry, hot environments. The high productivity of maize (Zea mays), sugarcane (Saccharum spp.) and several emerging bioenergy grasses is due largely to C(4) photosynthesis, which is enabled by the orderly arrangement, in concentric rings, of specialized bundle sheath and mesophyll cells in leaves in a pattern known as Kranz anatomy. Here we show that PIN, the auxin efflux protein, is present in the end walls of maize bundle sheath cells, as it is in the endodermis of the root. Since this marker suggests the expression of endodermal genetic programs in bundle sheath cells, we determined whether the transcription factor SCARECROW, which regulates structural differentiation of the root endodermis, also plays a role in the development of Kranz anatomy in maize. Mutations in the Scarecrow gene result in proliferation of bundle sheath cells, abnormal differentiation of bundle sheath chloroplasts, vein disorientation, loss of minor veins and reduction of vein density. Further characterization of this signal transduction pathway should facilitate the transfer of the C(4) trait into C(3) crop species, including rice.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Differentiation
  • Cell Proliferation
  • Cell Wall / metabolism
  • Chloroplasts / metabolism
  • Mesophyll Cells / ultrastructure
  • Mutation
  • Photosynthesis / physiology
  • Plant Leaves / genetics
  • Plant Leaves / growth & development*
  • Plant Leaves / physiology
  • Plant Leaves / ultrastructure
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Roots / physiology
  • Plant Roots / ultrastructure
  • Plants, Genetically Modified
  • Recombinant Fusion Proteins
  • Signal Transduction
  • Species Specificity
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Zea mays / genetics*
  • Zea mays / growth & development
  • Zea mays / physiology
  • Zea mays / ultrastructure

Substances

  • Plant Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors