S100A10 is required for the organization of actin stress fibers and promotion of cell spreading

Mol Cell Biochem. 2013 Feb;374(1-2):105-11. doi: 10.1007/s11010-012-1509-2. Epub 2012 Nov 6.


Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskeletal organization and cell spreading. Depletion of S100A10 induced disruption of stress fiber formation and delay in cell spreading. Rac1 activation during spreading was suppressed by S100A10 knockdown, and exogenous expression of active Rac1 restored the ability of cells to spread in the absence of S100A10. Our results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism*
  • Annexin A2 / genetics*
  • Annexin A2 / metabolism*
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Protein Transport
  • RNA Interference
  • RNA, Small Interfering
  • S100 Proteins / genetics*
  • S100 Proteins / metabolism*
  • Stress Fibers
  • rac1 GTP-Binding Protein / metabolism*


  • Annexin A2
  • RAC1 protein, human
  • RNA, Small Interfering
  • S100 Proteins
  • S100 calcium binding protein A10
  • rac1 GTP-Binding Protein