Genomic occupancy of the transcriptional co-activators p300 and CBP

Transcription. Jan-Feb 2013;4(1):18-23. doi: 10.4161/trns.22601. Epub 2012 Nov 6.

Abstract

The p300 and CBP co-activators are histone acetylases and central regulators of transcription in metazoans. The genomic occupancy of p300/CBP detected by ChIP-seq experiments can be used to identify transcriptional enhancers. However, studies in Drosophila embryos suggest that there is a preference for some transcription factors in directing p300/CBP to the genome. Although p300/CBP occupancy in general correlates with gene activation, they can also be found at silent genomic regions, which does not result in histone acetylation. Polycomb-mediated H3K27me3 is associated with repression, but does not preclude p300/CBP binding. An antagonism between H3K27ac and H3K27me3 indicates that p300/CBP may be involved in switching between repressed and active chromatin states.

Keywords: CBP; ChIP-seq; Polycomb; enhancer; p300; transcription.

Publication types

  • Review

MeSH terms

  • Acetylation
  • Animals
  • CREB-Binding Protein / metabolism*
  • E1A-Associated p300 Protein / metabolism*
  • Enhancer Elements, Genetic
  • Gene Expression Regulation
  • Gene Silencing
  • Genome*
  • Histones / metabolism
  • Humans
  • Polycomb-Group Proteins / metabolism
  • Protein Binding
  • Regulatory Sequences, Nucleic Acid
  • Transcriptional Activation

Substances

  • Histones
  • Polycomb-Group Proteins
  • CREB-Binding Protein
  • E1A-Associated p300 Protein