Automated grid handling and image acquisition for two-dimensional crystal screening

Methods Mol Biol. 2013;955:297-305. doi: 10.1007/978-1-62703-176-9_16.

Abstract

Routine large-scale two-dimensional (2D) crystallization trials are good candidates for automation. For imaging the large number of grids prepared from 2D crystallization trials, a two-pass imaging protocol using Leginon and a grid handling robot is described. A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cryoelectron Microscopy / instrumentation
  • Cryoelectron Microscopy / methods*
  • Crystallography / instrumentation
  • Crystallography / methods*
  • Internet
  • Microscopy, Electron, Transmission / instrumentation
  • Microscopy, Electron, Transmission / methods
  • Robotics* / instrumentation
  • Robotics* / methods
  • Software