Processing of electron diffraction patterns with the XDP program

Methods Mol Biol. 2013;955:539-50. doi: 10.1007/978-1-62703-176-9_29.

Abstract

Electron crystallography using two-dimensional crystals of membrane protein can provide high-resolution structure of a membrane protein within a lipid bilayer. With this technique, it is advantageous to use electron diffraction patterns to collect accurate intensities of the structure factors at high resolution. Here we describe how to process diffraction patterns using the XDP program and show what parameters are used and how they are determined in the process. Finally, the procedures for merging the intensity data will be described briefly.

MeSH terms

  • Image Processing, Computer-Assisted / methods*
  • Membrane Proteins / chemistry*
  • Software*
  • X-Ray Diffraction / methods*

Substances

  • Membrane Proteins