Real-time monitoring of protein conformational dynamics in solution using kinetic capillary electrophoresis

Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12464-8. doi: 10.1002/anie.201205575. Epub 2012 Nov 6.

Abstract

Conformational analysis: Capillary electrophoresis (CE) allows for the rapid separation of slowly interconverting protein conformers. Kinetic analysis (k(open), k(closed), and K(d)) of electropherograms in the presence and absence of effector ligands allows the measurement of kinetic and thermodynamic constants associated with conformational changes and ligand binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allosteric Regulation
  • Calcium / chemistry
  • Electrophoresis, Capillary*
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / metabolism
  • Guanosine Diphosphate / chemistry
  • Guanosine Diphosphate / metabolism
  • Humans
  • Kinetics
  • Ligands
  • Protein Structure, Tertiary
  • Proteins / chemistry*
  • Proteins / metabolism
  • Solutions / chemistry*
  • Thermodynamics
  • Transglutaminases / chemistry
  • Transglutaminases / metabolism

Substances

  • Ligands
  • Proteins
  • Solutions
  • Guanosine Diphosphate
  • transglutaminase 2
  • Transglutaminases
  • GTP-Binding Proteins
  • Calcium