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. 2011 Dec;61(4):420-5.
doi: 10.1270/jsbbs.61.420. Epub 2011 Dec 15.

Strawberry Cultivar Identification Based on Hypervariable SSR Markers

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Free PMC article

Strawberry Cultivar Identification Based on Hypervariable SSR Markers

Masanori Honjo et al. Breed Sci. .
Free PMC article

Abstract

We genotyped strawberry cultivars by two newly selected and two previously reported SSR markers. All four markers produced interpretable electropherograms from 75 accessions consisting of 72 Fragaria × ananassa cultivars or lines and three octoploid Fragaria species accessions. These SSR markers were highly polymorphic; in particular, one of the newly developed markers, FxaHGA02P13, was capable of distinguishing all of the accessions except for a mutant strain that was derived from another accession in the set. When two markers were combined, all 48 full-sib individuals could be distinguished. Fingerprinting patterns were reproducible between multiple samples, including the leaves, sepals, and fruit flesh of the same accession. Principal-coordinate analysis of the 75 accessions detected several groups, which reflect taxon and breeding site. Together with other available markers, these SSR markers will contribute to the management of strawberry genetic resources and the protection of breeders' rights.

Keywords: Fragaria × ananassa; cultivated strawberry; fingerprinting; genotyping; microsatellite.

Figures

Fig. 1
Fig. 1
Amplified fragment patterns of SSR FxaHGA02P13 from different tissue samples of two cultivars. The number above each peak indicates the fragment size.
Fig. 2
Fig. 2
Principal-coordinate (PCO) analysis of 75 accessions consisting of 72 F. × ananassa cultivars or lines and three octoploid Fragaria species accessions analyzed by four SSR markers. The first (PCO1) and second (PCO2) axes explain 11.6% and 7.3% of the total variation, respectively. Numbers correspond to accession numbers in Table 1.

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