Zebrafish teratogenicity testing

Methods Mol Biol. 2013;947:383-401. doi: 10.1007/978-1-62703-131-8_28.

Abstract

As a model for teratogenicity research, zebrafish are gaining popularity and creditability. Zebrafish embryos have been proven to be a highly valuable tool in genetics and developmental biology research and have advanced our understanding of a number of known developmental toxicants. It has yet to be determined conclusively how reliably a zebrafish embryo screening assay predicts what will happen in mammalian models, but results from initial assessments have been encouraging. Here we have presented procedures for the basic care of a zebrafish colony to support embryo production, embryo collection and culturing, and teratogenicity experiments.

MeSH terms

  • Animal Husbandry
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian / abnormalities
  • Embryo, Nonmammalian / drug effects
  • Environment, Controlled
  • Female
  • Gene Expression Regulation, Developmental / drug effects
  • Injections
  • Male
  • Morpholinos / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Teratology / methods*
  • Toxicity Tests / methods*
  • Zebrafish / abnormalities*
  • Zebrafish / embryology*
  • Zebrafish / genetics

Substances

  • Morpholinos
  • RNA, Messenger