Diet enriched with korean pine nut oil improves mitochondrial oxidative metabolism in skeletal muscle and brown adipose tissue in diet-induced obesity

J Agric Food Chem. 2012 Dec 5;60(48):11935-41. doi: 10.1021/jf303548k. Epub 2012 Nov 26.

Abstract

In this study, we investigated effects of pine nut oil (PNO) on high-fat-diet (HFD)-induced obesity and metabolic dysfunction in skeletal muscle and brown adipose tissue (BAT). Male C57BL/6 mice were fed a HFD with 15% energy from lard and 30% energy from either soybean oil (SBO-HFD) or PNO (PNO-HFD) for 12 weeks. The PNO-HFD resulted in less weight gain and intramuscular lipid accumulation than the SBO-HFD and was accompanied by upregulation of transcripts and proteins related to oxidative metabolism and phosphorylation of AMP-activated protein kinase (AMPK), as well as molecules selectively expressed in type I and type IIa muscle fibers. In addition, uncoupling protein-1 was upregulated in BAT. These beneficial metabolic effects were partly associated with the dual ligand activity of pinolenic acid, which is abundant in PNO, for peroxisome proliferator-activated receptors α and δ. Our findings suggest that PNO may have potential as a dietary supplement for counteracting obesity and metabolic dysregulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases / metabolism
  • Adipose Tissue, Brown / drug effects
  • Adipose Tissue, Brown / metabolism*
  • Animals
  • Diet
  • Diet, High-Fat / adverse effects
  • Dietary Fats / pharmacology
  • Ion Channels / metabolism
  • Linolenic Acids / pharmacology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • Mitochondrial Proteins / metabolism
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / metabolism*
  • Nuts / chemistry
  • Obesity / chemically induced
  • Obesity / diet therapy
  • Obesity / metabolism*
  • PPAR alpha / metabolism
  • PPAR delta / metabolism
  • Phosphorylation / drug effects
  • Pinus / chemistry*
  • Plant Oils / pharmacology*
  • Soybean Oil / pharmacology
  • Thermogenesis / drug effects
  • Uncoupling Protein 1
  • Weight Gain / drug effects

Substances

  • Dietary Fats
  • Ion Channels
  • Linolenic Acids
  • Mitochondrial Proteins
  • PPAR alpha
  • PPAR delta
  • Plant Oils
  • Ucp1 protein, mouse
  • Uncoupling Protein 1
  • 5,9,12-octadecatrienoic acid
  • Soybean Oil
  • AMP-Activated Protein Kinases
  • lard