Designer nucleic acids to probe and program the cell

Trends Cell Biol. 2012 Dec;22(12):624-33. doi: 10.1016/j.tcb.2012.10.001. Epub 2012 Nov 8.


Recent advances in nucleic acid sequencing, structural, and computational technologies have resulted in dramatic progress in our understanding of nucleic acid structure and function in the cell. This knowledge, together with the predictable base-pairing of nucleic acids and powerful synthesis and expression capabilities now offers the unique ability to program nucleic acids to form precise 3D architectures with diverse applications in synthetic and cell biology. The unique modularity of structural motifs that include aptamers, DNAzymes, and ribozymes, together with their well-defined construction rules, enables the synthesis of functional higher-order nucleic acid complexes from these subcomponents. As we illustrate here, these highly programmable, smart complexes are increasingly enabling researchers to probe and program the cell in a sophisticated manner that moves well beyond the use of nucleic acids for conventional genetic manipulation alone.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Bacteria / chemistry
  • Bacteria / genetics
  • Cell Nucleus / chemistry
  • Cell Nucleus / genetics
  • Cellular Reprogramming*
  • DNA / chemistry*
  • DNA / genetics
  • Gene Targeting / methods*
  • Nanostructures
  • Nanotechnology / methods*
  • Nucleic Acid Conformation
  • Nucleotide Motifs
  • Protein Biosynthesis
  • RNA / chemistry*
  • RNA / genetics
  • RNA Probes / chemistry
  • RNA Transport
  • Synthetic Biology


  • RNA Probes
  • RNA
  • DNA