Systematic reconstruction of RNA functional motifs with high-throughput microfluidics

Nat Methods. 2012 Dec;9(12):1192-4. doi: 10.1038/nmeth.2225. Epub 2012 Nov 11.


We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Gene Library
  • Glutathione Transferase
  • Humans
  • Microfluidics / methods*
  • Nucleotide Motifs*
  • Phylogeny
  • RNA / chemistry*
  • RNA Probes


  • RNA Probes
  • RNA
  • Glutathione Transferase