BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

Biotechnol Lett. 2013 Mar;35(3):301-8. doi: 10.1007/s10529-012-1084-3. Epub 2012 Nov 10.


Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow*
  • Bone Morphogenetic Protein 2 / metabolism*
  • Cell Culture Techniques
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Drug Synergism
  • Fibroblast Growth Factors / metabolism*
  • Mesenchymal Stem Cells / physiology*
  • Rats
  • Vascular Endothelial Growth Factor A / metabolism*


  • Bone Morphogenetic Protein 2
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • Fibroblast Growth Factors