The RNA helicase DDX5/p68 is a key factor promoting c-fos expression at different levels from transcription to mRNA export

Nucleic Acids Res. 2013 Jan 7;41(1):554-64. doi: 10.1093/nar/gks1046. Epub 2012 Nov 11.


It is widely accepted that pre-mRNA maturation, including splicing, is tightly coupled to both transcription and mRNA export, but factors linking the three processes are less understood. By analysing the estrogen-regulated expression of the c-fos mRNA that is processed during transcription, we show that the ddx5 RNA helicase, is required throughout the major nuclear steps of the expression of the c-fos gene, from transcription to mRNA export. Indeed, ddx5, whose recruitment on the c-fos gene was increased upon estrogen treatment, was required for the full transcriptional activation of the c-fos gene. In addition, ddx5 was required for c-fos co-transcriptional RNA splicing. When splicing occurred post-transcriptionally in the absence of ddx5, the c-fos mRNA was poorly exported into the cytosol because of inefficient recruitment of the TAP mRNA export receptor. Finally, ddx5 was present in the c-fos messenger ribonucleoprotein together with mRNA export factors, which further supports that ddx5 is a key operator in the c-fos 'mRNA factory'.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • DEAD-box RNA Helicases / physiology*
  • Estradiol / pharmacology
  • Humans
  • MCF-7 Cells
  • Proto-Oncogene Proteins c-fos / biosynthesis
  • Proto-Oncogene Proteins c-fos / genetics*
  • Proto-Oncogene Proteins c-fos / metabolism
  • RNA Splicing
  • RNA Transport
  • RNA, Messenger / metabolism*
  • Ribonucleoproteins / metabolism
  • Transcription, Genetic
  • Transcriptional Activation*


  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger
  • Ribonucleoproteins
  • messenger ribonucleoprotein
  • Estradiol
  • Ddx5 protein, human
  • DEAD-box RNA Helicases