Clostridium Carboxidivorans Strain P7T Recombinant Formate Dehydrogenase Catalyzes Reduction of CO(2) to Formate

Appl Environ Microbiol. 2013 Jan;79(2):741-4. doi: 10.1128/AEM.02886-12. Epub 2012 Nov 9.

Abstract

Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7(T), expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4°C under oxic conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Dioxide / metabolism*
  • Cloning, Molecular
  • Clostridium / enzymology*
  • Clostridium / genetics
  • Enzyme Stability
  • Escherichia coli / genetics
  • Formate Dehydrogenases / chemistry
  • Formate Dehydrogenases / genetics
  • Formate Dehydrogenases / isolation & purification
  • Formate Dehydrogenases / metabolism*
  • Formates / metabolism*
  • Gene Expression
  • Kinetics
  • Oxidation-Reduction
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Temperature
  • Time Factors

Substances

  • Formates
  • Recombinant Proteins
  • formic acid
  • Carbon Dioxide
  • Formate Dehydrogenases