Grape seed extract as a potential remineralizing agent: a comparative in vitro study

J Contemp Dent Pract. 2012 Jul 1;13(4):425-30. doi: 10.5005/jp-journals-10024-1162.


Objective: Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation.

Materials and methods: Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05).

Results: GSE revealed less demineralization and more remineralization compared with other groups.

Conclusion: GSE promotes remineralization of artificial root caries lesions.

Clinical significance: The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium.

Publication types

  • Comparative Study

MeSH terms

  • Analysis of Variance
  • Antioxidants / therapeutic use*
  • Fluorides / therapeutic use
  • Glycerophosphates / therapeutic use
  • Grape Seed Extract / therapeutic use*
  • Humans
  • Microscopy, Confocal
  • Phosphates / therapeutic use
  • Proanthocyanidins / therapeutic use*
  • Root Caries / drug therapy*
  • Tooth Remineralization / methods*


  • Antioxidants
  • Glycerophosphates
  • Grape Seed Extract
  • Phosphates
  • Proanthocyanidins
  • fluorophosphate
  • proanthocyanidin
  • Fluorides