In-depth Membrane Proteomic Study of Breast Cancer Tissues for the Generation of a Chromosome-Based Protein List

J Proteome Res. 2013 Jan 4;12(1):208-13. doi: 10.1021/pr300824m. Epub 2012 Nov 15.

Abstract

The Chromosome-centric Human Proteome Project (C-HPP) aims to define all proteins encoded in each chromosome and especially to identify proteins that currently lack evidence by mass spectrometry. The C-HPP also prioritizes particular protein subsets such as membrane proteins, post-translational modifications, and low-abundance proteins. In this study, we aimed to generate deep profiling of the membrane proteins of human breast cancer tissues on a chromosome-by-chromosome basis using shotgun proteomics. We identified 7092 unique proteins using membrane fractions isolated from pooled breast cancer tissues with high confidence. A total of 3282 proteins were annotated as membrane proteins by Gene Ontology analysis, which covered 45% of the membrane proteins predicted in 20,859 protein-coding genes. Furthermore, we were able to identify 851 membrane proteins that currently lack evidence by mass spectrometry in neXtProt. Our results will contribute to the accomplishment of the primary goal of the C-HPP in identifying so-called "missing proteins" and generating a whole protein catalog for each chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms* / genetics
  • Breast Neoplasms* / metabolism
  • Chromosomes, Human / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genome, Human
  • Humans
  • Mass Spectrometry
  • Membrane Proteins* / classification
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Protein Processing, Post-Translational
  • Proteomics*

Substances

  • Membrane Proteins