Identification of CCDC6-RET fusion in the human lung adenocarcinoma cell line, LC-2/ad

J Thorac Oncol. 2012 Dec;7(12):1872-1876. doi: 10.1097/JTO.0b013e3182721ed1.

Abstract

Rearranged during transfection (RET) fusions have been newly identified in approximately 1% of patients with primary lung tumors. However, patient-derived lung cancer cell lines harboring RET fusions have not yet been established or identified, and therefore, the effectiveness of an RET inhibitor on lung tumors with endogenous RET fusion has not yet been studied. In this study, we report identification of CCDC6-RET fusion in the human lung adenocarcinoma cell line LC-2/ad. LC-2/ad showed distinctive sensitivity to the RET inhibitor, vandetanib, among 39 non-small lung cancer cell lines. The xenograft tumor of LC-2/ad showed cribriform acinar structures, a morphologic feature of primary RET fusion-positive lung adenocarcinomas. LC-2/ad cells could provide useful resources to analyze molecular functions of RET-fusion protein and its response to RET inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / drug therapy
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Animals
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology
  • Blotting, Western
  • Carcinoma, Non-Small-Cell Lung / drug therapy
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cell Proliferation / drug effects
  • Cytoskeletal Proteins / genetics*
  • Female
  • Gefitinib
  • Humans
  • Immunoenzyme Techniques
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Oncogene Proteins, Fusion / genetics*
  • Phosphorylation / drug effects
  • Piperidines / administration & dosage
  • Proto-Oncogene Proteins c-ret / genetics*
  • Quinazolines / administration & dosage
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transplantation, Heterologous
  • Tumor Cells, Cultured

Substances

  • CCDC6 protein, human
  • Cytoskeletal Proteins
  • Oncogene Proteins, Fusion
  • Piperidines
  • Quinazolines
  • RNA, Messenger
  • Proto-Oncogene Proteins c-ret
  • RET protein, human
  • Gefitinib
  • N-(4-bromo-2-fluorophenyl)-6-methoxy-7-((1-methylpiperidin-4-yl)methoxy)quinazolin-4-amine