Phytochemical Analysis and Cytotoxicity Towards Multidrug-Resistant Leukemia Cells of Essential Oils Derived From Lebanese Medicinal Plants

Planta Med. 2012 Dec;78(18):1927-31. doi: 10.1055/s-0032-1327896. Epub 2012 Nov 15.

Abstract

Juniperus excelsa fruit essential oil as well as J. oxycedrus, Cedrus libani, and Pinus pinea wood essential oils have been obtained with yields between 2.2 ± 0.3 % to 3.4 ± 0.5 % and analyzed by gas chromatography. Sesquiterpenes mainly characterized C. libani and J. oxycedrus essential oils, while in P. pinea and J. excelsa, monoterpenes were the most abundant compounds. In J. oxycedrus, cis-calamenene (7.8 %), cuparene (3.8 %), and cis-thujopsenal (2.0 %) have been detected for the first time. The cytotoxic activity of these essential oils against drug-sensitive CCRF-CEM and multidrug-resistant P-glycoprotein-expressing CEM/ADR5000 leukemia cells has been investigated (IC₅₀ values: 29.46 to 61.54 µg/mL). Remarkably, multidrug-resistant CEM/ADR5000 cells did not reveal cross-resistance, indicating that these essential oils might be useful to treat otherwise drug-resistant and refractory tumors.

Publication types

  • Letter

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cedrus / chemistry
  • Drug Resistance, Multiple / drug effects
  • Drug Resistance, Neoplasm / drug effects*
  • Fruit / chemistry
  • Humans
  • Juniperus / chemistry
  • Lebanon
  • Leukemia / drug therapy*
  • Monoterpenes / isolation & purification
  • Oils, Volatile / chemistry*
  • Oils, Volatile / pharmacology
  • Phytotherapy*
  • Pinus / chemistry
  • Plants, Medicinal / chemistry*
  • Sesquiterpenes / isolation & purification
  • Tumor Cells, Cultured / drug effects*
  • Wood / chemistry

Substances

  • Antineoplastic Agents, Phytogenic
  • Monoterpenes
  • Oils, Volatile
  • Sesquiterpenes