Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Genes Dev. 2012 Nov 15;26(22):2477-82. doi: 10.1101/gad.202242.112.


Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15(INK4b), a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Proliferation
  • Female
  • Gene Expression Regulation, Developmental*
  • Genes, cdc / genetics*
  • Germ Cells / cytology*
  • Germ Cells / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Nuclear Proteins / metabolism
  • Positive Transcriptional Elongation Factor B / metabolism
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism
  • Ribonucleoproteins, Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / metabolism
  • Transcription Factors / metabolism


  • Brd4 protein, mouse
  • Nuclear Proteins
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Transcription Factors
  • Positive Transcriptional Elongation Factor B