A change pattern in enzyme release function of digestive glands exerts the organism as a whole. Dysfunctions of hydrolysis and intestinal malabsorption (secondary malabsorption syndrome) are the first step towards nutrition and metabolism processes abnormality and that can play a role in genesis of pathological conditions. Recent researches arouse clinicians interest in determination of biofluid enzyme activity in different physiological and abnormal cases. Intestinal infections are followed by dysbacteriosis and obvious alterations in the hydrolase levels in the blood, urine and motions because of the changes of functional status of the liver, renal and intestinal barriers in relation to increted digestive enzymes. This causes an unfavorable course of recovery with the development of postinfectious digestion disorders as a result of previous acute diarrhea. Future researches are necessary to elaborate appropriate remodeling of developed pathosis with the help of enzymotherapy and probiotic diet.