Identification of phenolic constituents in Cichorium endivia var. crispum and var. latifolium salads by high-performance liquid chromatography with diode array detection and electrospray ioniziation tandem mass spectrometry

J Agric Food Chem. 2012 Dec 12;60(49):12142-50. doi: 10.1021/jf3034754. Epub 2012 Nov 29.


Chicory is a widely consumed vegetable and a source of phenolic compounds. Phenolic acid and flavonoid derivatives were identified in Cichorium endivia var. crispum and var. latifolium and fully characterized using complementary information from two different high-performance liquid chromatography detectors, diode array and mass spectrometer, in positive and negative modes. We describe about 40 phenolic compounds, some of which have never previously been reported in these plants, such as hydroxycinnamic acid derivatives (i.e., different mono- and dicaffeoylquinic acid isomers) and mono- and diglycosides of quercetin, kaempferol, and myricetin (differing also by the glycosylation site). These data provide a contribution to a more exhaustive identification of phenolic compounds in C. endivia vegetables.

MeSH terms

  • Chicory / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Coumaric Acids / analysis
  • Flavonoids / analysis
  • Flavonols / analysis
  • Flavonols / chemistry
  • Food Analysis / methods*
  • Isomerism
  • Kaempferols / analysis
  • Phenols / analysis*
  • Phenols / chemistry
  • Quercetin / analysis
  • Quinic Acid / analogs & derivatives
  • Quinic Acid / analysis
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*


  • Coumaric Acids
  • Flavonoids
  • Flavonols
  • Kaempferols
  • Phenols
  • caffeoylquinic acid
  • Quinic Acid
  • kaempferol
  • myricetin
  • Quercetin