HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3

Virology. 2013 Jan 20;435(2):329-40. doi: 10.1016/j.virol.2012.10.032. Epub 2012 Nov 13.


Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus*
  • Gene Expression Regulation, Viral
  • HIV-1 / genetics
  • HIV-1 / metabolism*
  • Humans
  • Nuclear Matrix-Associated Proteins / genetics
  • Nuclear Matrix-Associated Proteins / metabolism*
  • PTB-Associated Splicing Factor
  • RNA Precursors / genetics*
  • RNA Precursors / metabolism
  • RNA Processing, Post-Transcriptional
  • RNA, Viral / genetics
  • RNA, Viral / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • rev Gene Products, Human Immunodeficiency Virus / genetics
  • rev Gene Products, Human Immunodeficiency Virus / metabolism*


  • MATR3 protein, human
  • Nuclear Matrix-Associated Proteins
  • PTB-Associated Splicing Factor
  • RNA Precursors
  • RNA, Viral
  • RNA-Binding Proteins
  • rev Gene Products, Human Immunodeficiency Virus