Aeromonas australiensis sp. nov., isolated from irrigation water

Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2270-2276. doi: 10.1099/ijs.0.040162-0. Epub 2012 Nov 16.

Abstract

A Gram-negative, facultatively anaerobic bacillus, designated strain 266(T), was isolated from an irrigation water system in the south-west of Western Australia. Analysis of the 16S rRNA gene sequence confirmed that strain 266(T) belonged to the genus Aeromonas, with the nearest species being Aeromonas fluvialis (99.6% similarity to the type strain, with 6 nucleotide differences) followed by Aeromonas veronii and Aeromonas allosaccharophila (both 99.5%). Analysis of gyrB and rpoD sequences suggested that strain 266(T) formed a phylogenetic line independent of other species in the genus. This was confirmed using the concatenated sequences of six housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA and dnaX) that also indicated that A. veronii and A. allosaccharophila were the nearest relatives. DNA-DNA reassociation experiments and phenotypic analysis further supported the conclusion that strain 266(T) represents a novel species, for which the name Aeromonas australiensis sp. nov. is proposed, with type strain 266(T) (=CECT 8023(T) =LMG 26707(T)). [corrected].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas / classification*
  • Aeromonas / genetics
  • Aeromonas / isolation & purification
  • Agricultural Irrigation
  • Bacterial Typing Techniques
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Genes, Bacterial
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Water Microbiology*
  • Western Australia

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/FN691773
  • GENBANK/FN773335
  • GENBANK/HE611951
  • GENBANK/HE611952
  • GENBANK/HE611953
  • GENBANK/HE611954
  • GENBANK/HE611955