Imaging mass spectrometry and genome mining reveal highly antifungal virulence factor of mushroom soft rot pathogen

Angew Chem Int Ed Engl. 2012 Dec 21;51(52):13173-7. doi: 10.1002/anie.201206658. Epub 2012 Nov 19.

Abstract

Caught in the act: imaging mass spectrometry of a button mushroom infected with the soft rot pathogen Janthinobacterium agaricidamnosum in conjunction with genome mining revealed jagaricin as a highly antifungal virulence factor that is not produced under standard cultivation conditions. The structure of jagaricin was rigorously elucidated by a combination of physicochemical analyses, chemical derivatization, and bioinformatics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / growth & development
  • Antifungal Agents / chemistry
  • Antifungal Agents / metabolism
  • Antifungal Agents / pharmacology
  • Computational Biology
  • Fungi / drug effects
  • Genome, Bacterial*
  • Gram-Negative Bacteria / genetics*
  • Gram-Negative Bacteria / isolation & purification
  • Magnetic Resonance Spectroscopy
  • Molecular Conformation
  • Peptide Synthases / genetics
  • Peptide Synthases / metabolism
  • Peptides, Cyclic / chemical synthesis*
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / pharmacology
  • Plant Roots / microbiology
  • Polyketide Synthases / genetics
  • Polyketide Synthases / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Antifungal Agents
  • Peptides, Cyclic
  • jagaricin
  • Polyketide Synthases
  • Peptide Synthases
  • non-ribosomal peptide synthase