Betula pendula Roth leaves: gastroprotective effects of an HPLC-fingerprinted methanolic extract

Nat Prod Res. 2013;27(17):1569-75. doi: 10.1080/14786419.2012.740036. Epub 2012 Nov 19.

Abstract

In this study, a methanolic extract of Betula pendula leaves (BLE) was investigated for its gastroprotective effects against 90% ethanol-induced ulcer in rats. Oral pretreatment of rats with BLE (100, 200 and 400 mg kg(- 1)) significantly reduced the incidence of gastric lesions induced by ethanol administration as compared with misoprostol (0.50 mg kg(- 1)). Furthermore, BLE inhibited the increase in malondialdehyde (MDA) and prevented depletion of total sulhydryl and non-protein sulhydryl groups in rat stomach homogenate when compared with ethanol group. With regard to the effect of lipid peroxidation in vitro, BLE showed the ability to reduce methyl linoleate autoxidation. Chemical characterisation of the main biologically active constituents of BLE was also achieved by means of high-performance liquid chromatography with photodiode array and mass spectrometry detection, showing the presence of myricetin-3-O-galactoside, quercetin glycosides, kaempferol glycosides.

MeSH terms

  • Animals
  • Anti-Ulcer Agents / chemistry
  • Anti-Ulcer Agents / therapeutic use
  • Betula / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Galactosides / chemistry
  • Galactosides / therapeutic use
  • Gastric Mucosa / drug effects
  • Kaempferols / chemistry
  • Kaempferols / therapeutic use
  • Lipid Peroxidation / drug effects
  • Malondialdehyde / metabolism
  • Methanol / chemistry*
  • Plant Extracts / chemistry*
  • Plant Extracts / therapeutic use
  • Plant Leaves / chemistry*
  • Rats
  • Stomach Ulcer / chemically induced
  • Stomach Ulcer / prevention & control

Substances

  • Anti-Ulcer Agents
  • Galactosides
  • Kaempferols
  • Plant Extracts
  • myricetin-3-O-galactoside
  • Malondialdehyde
  • kaempferol
  • Methanol