Transcription regulation of a yeast gene from a downstream location

J Mol Biol. 2013 Feb 8;425(3):457-65. doi: 10.1016/j.jmb.2012.11.018. Epub 2012 Nov 20.


Mechanisms for coregulation of transcription of tandem genes in yeast remain largely speculative. This study focused on inositol-mediated regulation of the tandem gene pair SNA3-INO1. While the pattern of regulation of these two genes was similar, results showed that intermediate levels of inositol repressed INO1 and induced SNA3. Results also showed that inositol-mediated regulation of the SNA3 gene was not a function of its promoter but occurred from factors within the SNA3-INO1 intergenic region. The basic helix-loop-helix proteins, Ino2p and Ino4p, mediated this regulation through the upstream activation sequence (UAS)(INO) (E-box) sequences in the intergenic region. These results provide a model for studying coregulation of yeast tandem genes. This is especially significant given that many tandem gene pairs in yeast are coregulated even though context-specific UAS sequences are known only for one gene in the pair.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Gene Expression Regulation, Fungal*
  • Inositol / metabolism
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Myo-Inositol-1-Phosphate Synthase / biosynthesis*
  • Myo-Inositol-1-Phosphate Synthase / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / biosynthesis*
  • Saccharomyces cerevisiae Proteins / genetics
  • Transcription, Genetic*


  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Sna3 protein, S cerevisiae
  • Inositol
  • INO1 protein, S cerevisiae
  • Myo-Inositol-1-Phosphate Synthase