The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli

Biochem Soc Trans. 2012 Dec 1;40(6):1222-6. doi: 10.1042/BST20120143.


Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cloning, Molecular*
  • DNA Transposable Elements
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Bacterial
  • Genetic Engineering
  • Mutagenesis, Insertional
  • Promoter Regions, Genetic
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Transformation, Bacterial


  • DNA Transposable Elements
  • Recombinant Proteins