Expression of human chromosome 2 ornithine decarboxylase gene in ornithine decarboxylase-deficient Chinese hamster ovary cells

Cancer Res. 1990 Apr 15;50(8):2239-44.


Ornithine decarboxylase (ODC) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and ODC is essential for cell proliferation. After screening 2.5 X 10(5) plaques using a human ODC complementary DNA probe, a typical clone with a 5.4-kilobase insert was isolated and then cloned into the HindIII site of the pGem-1 vector. One (phODC 2B1) of these clones containing a 5.4-kilobase ODC gene insert was identified. Restriction enzyme analysis and partial sequencing data revealed that phODC 2B1 contained the full length protein-coding sequences but lacked first exon and 3'-polyadenylation sequences. Primer extension analysis indicated that human ODC mRNA has homologous sequences with the ODC gene from human chromosome 2. To determine that the chromosome 2 ODC gene is functional, ODC-deficient Chinese hamster ovary cells were transfected with the ODC expression vector (phSV2B1-neo) and several G418-resistant transfectants were isolated which expressed 70- to 400-fold more ODC activity than parental or wild-type Chinese hamster ovary cells. Furthermore, these stable transfectants exhibited a higher growth rate than wild-type cells. These results indicate that the ODC gene from human chromosome 2 encodes functional ODC protein, and ODC (and its product putrescine) is required for cell growth.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Cycle
  • Cell Division
  • Cell Line
  • Chromosomes, Human, Pair 2*
  • Cricetinae
  • Cricetulus
  • Female
  • Gene Expression*
  • Genes*
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Multigene Family
  • Oligonucleotide Probes
  • Ornithine Decarboxylase / deficiency
  • Ornithine Decarboxylase / genetics*
  • Ornithine Decarboxylase / metabolism
  • Ovary
  • Plasmids
  • Polyamines / metabolism
  • Restriction Mapping
  • Transfection*


  • Oligonucleotide Probes
  • Polyamines
  • Ornithine Decarboxylase