Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs

Dev Comp Immunol. 2013 Apr;39(4):409-18. doi: 10.1016/j.dci.2012.10.010. Epub 2012 Nov 23.

Abstract

Monoclonal antibodies IAH-CC51, BB6-11C9.6 and B-Ly4 are routinely used to detect CD21 orthologue on the surface of porcine B lymphocytes. Cross-reactive studies show that IAH-CC51 and B-Ly4 recognize only a portion of B cells that are positive for pan-specific BB6-11C9.6. This indicates that CD21 is always present on all mature B cells but can be expressed in at least two differential forms, and these were assigned as CD21(a) and CD21(b). We used IAH-CC51 together with anti-CD2 to define four subpopulations of B cells. Ontogenetic and in vitro culture studies, analysis of cell size, expression of CD11b and class-switched phenotype together with measurement of proliferation and cell death, revealed that these subsets represent distinct populations. Phenotypic and functional features collectively suggest that CD21(b+) B cells are less mature than CD21(b-). The present work is the first to show that distinct subsets of mature B cells can express differential forms of CD21.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Apoptosis
  • B-Lymphocyte Subsets / classification
  • B-Lymphocyte Subsets / immunology*
  • Cells, Cultured
  • Epitopes / immunology
  • Receptors, Complement 3d / immunology*
  • Swine / immunology*

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Receptors, Complement 3d