Nonrandom induction of pyrimidine-pyrimidone (6-4) photoproducts in ultraviolet-irradiated human chromatin

J Biol Chem. 1990 Apr 5;265(10):5353-6.

Abstract

Radioimmunoassays that detect pyrimidine-pyrimidone (6-4) photoproducts and cyclobutane dimers were used to determine the relative induction of these photoproducts in nucleosomal (core) and internucleosomal (linker) DNA in human cell chromatin irradiated with UV light. Cyclobutane dimers were formed in equal amounts/nucleotide in core and linker DNA, whereas (6-4) photoproducts occurred with 6-fold greater frequency/nucleotide in linker DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Chromatin / metabolism
  • Chromatin / radiation effects*
  • DNA / metabolism
  • DNA / radiation effects
  • Humans
  • Nucleosomes / metabolism
  • Photochemistry
  • Pyrimidine Dimers / metabolism*
  • Radioimmunoassay
  • Ultraviolet Rays*

Substances

  • Chromatin
  • Nucleosomes
  • Pyrimidine Dimers
  • pyrimidine-pyrimidone dimer
  • DNA