The flavin-containing monooxygenase enzymes expressed in rabbit liver and lung are products of related but distinctly different genes

J Biol Chem. 1990 Apr 5;265(10):5855-61.


Preparations of mRNA isolated from rabbit lung and liver were used in the construction of libraries that were screened for cDNAs encoding the pulmonary or hepatic isozyme of the flavin-containing monooxygenase. The hepatic library was screened with cDNA encoding the flavin-containing monooxygenase expressed in pig liver, and a clone containing a 2.0-kilobase insert was detected and isolated. This cDNA insert encoded a protein of 535 amino acids with a primary structure 87% identical to that of the pig flavin-containing monooxygenase. The pulmonary library was screened with polyclonal antibodies to the flavin-containing monooxygenase expressed in rabbit lung, and a clone containing a 2.6-kilobase insert was detected and isolated. Although the protein encoded by this insert also contained 535 amino acids, its primary sequence was only 56% identical to that of the liver enzyme. The sequences of several peptides obtained by digestion of the purified rabbit pulmonary flavin-containing monooxygenase with trypsin matched exactly with sequences derived from the cDNA structure. Tissue-specific distribution of mRNA for the hepatic and pulmonary isozymes of the flavin-containing monooxygenase was consistent with the distribution of protein, an indication that expression of flavin-containing monooxygenase is controlled at the level of transcription. Analysis of genomic DNA indicates that both the hepatic and pulmonary enzymes may be products of single genes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • DNA / genetics
  • DNA / isolation & purification
  • Flavin-Adenine Dinucleotide / metabolism
  • Gene Expression*
  • Isoenzymes / genetics*
  • Kidney / analysis
  • Liver / analysis*
  • Lung / analysis*
  • Molecular Sequence Data
  • NADP / metabolism
  • Nucleic Acid Hybridization
  • Oxygenases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Rabbits
  • Sequence Homology, Nucleic Acid
  • Swine
  • Tissue Distribution


  • Isoenzymes
  • RNA, Messenger
  • Flavin-Adenine Dinucleotide
  • NADP
  • DNA
  • Oxygenases
  • dimethylaniline monooxygenase (N-oxide forming)

Associated data

  • GENBANK/J05275
  • GENBANK/M32029
  • GENBANK/M32030