Perhydrolase-nanotube paint composites with sporicidal and antiviral activity

Appl Microbiol Biotechnol. 2013 Oct;97(19):8813-21. doi: 10.1007/s00253-012-4573-3. Epub 2012 Nov 28.


AcT (perhydrolase) containing paint composites were prepared leading to broad-spectrum decontamination. AcT was immobilized onto multi-walled carbon nanotubes (MWNTs) and then incorporated into latex-based paints to form catalytic coatings. These AcT-based paint composites showed a 6-log reduction in the viability of spores of Bacillus cereus and Bacillus anthracis (Sterne) within 60 min. The paint composites also showed >4-log reduction in the titer of influenza virus (X-31) within 10 min (initially challenged with 10(7) PFU/mL). AcT-based paint composites were also tested using various perhydrolase acyl donor substrates, including propylene glycol diacetate (PGD), glyceryl triacetate, and ethyl acetate, with PGD observed to be the best among the substrates tested for generation of peracetic acid and killing of bacillus spores. The operational stability of paint composites was also studied at different relative humidities and temperatures to simulate real-life operation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / metabolism*
  • Antiviral Agents / metabolism*
  • Bacillus anthracis / drug effects
  • Bacillus anthracis / physiology
  • Bacillus cereus / drug effects
  • Bacillus cereus / physiology
  • Enzymes, Immobilized / metabolism*
  • Hydrolases / metabolism*
  • Microbial Viability / drug effects*
  • Nanotubes*
  • Orthomyxoviridae / drug effects
  • Paint
  • Peracetic Acid / metabolism
  • Spores, Bacterial / drug effects
  • Spores, Bacterial / physiology


  • Anti-Infective Agents
  • Antiviral Agents
  • Enzymes, Immobilized
  • Hydrolases
  • Peracetic Acid