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Antimicrobial Effectiveness of Silver Nanoparticles Co-Stabilized by the Bioactive Copolymer Pluronic F68

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Antimicrobial Effectiveness of Silver Nanoparticles Co-Stabilized by the Bioactive Copolymer Pluronic F68

Carolina Alves dos Santos et al. J Nanobiotechnology.

Abstract

Background: Silver nanoparticles (AgNps) have attracted much interest in biomedical engineering, since they have excellent antimicrobial properties. Therefore, AgNps have often been considered for incorporation into medical products for skin pathologies to reduce the risk of contamination. This study aims at evaluating the antimicrobial effectiveness of AgNps stabilized by pluronic™ F68 associated with other polymers such as polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP).

Methods: AgNps antimicrobial activity was evaluated using the minimum inhibitory concentration (MIC) method. The action spectrum was evaluated for different polymers associated with pluronic™ F68 against the gram negative bacteria P. aeuroginosa and E. coli and the gram positive bacteria S. Aureus.

Results: AgNps stabilized with PVP or PVA and co-stabilized with pluronic™ F68 are effective against E. coli and P. aeruginosa microorganisms, with MIC values as low as 0.78% of the concentration of the original AgNps dispersion. The antimicrobial action against S. aureus is poor, with MIC values not lower than 25%.

Conclusions: AgNps stabilized by different polymeric systems have shown improved antimicrobial activity against gram-negative microorganisms in comparison to unstabilized AgNps. Co-stabilization with the bioactive copolymer pluronic™ F68 has further enhanced the antimicrobial effectiveness against both microorganisms. A poor effectiveness has been found against the gram-positive S. aureus microorganism. Future assays are being delineated targeting possible therapeutic applications.

Figures

Figure 1
Figure 1
Minimum Inhibitory Concentration Methodology (MIC).

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References

    1. Ragaseema VM, Unnikrishnan S, Kalliyana Krishnan V, Krishnan Lissy K. The antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces. Biomaterials. 2012;33:3083–3092. doi: 10.1016/j.biomaterials.2012.01.005. - DOI - PubMed
    1. Furno F, Morley KS, Wong B, Sharp BL, Arnold PL, Howdle SM, Bayston R, Brown PD, Winship PD, Reid HJ. Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection? J Antimicrob Chemother. 2004;54:1019–1024. doi: 10.1093/jac/dkh478. - DOI - PubMed
    1. Irwin P, Martin J, Nguyen LH, He Y, Gehring A, Chen CY. Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. J Nanobiotechnology. 2010;8:34. doi: 10.1186/1477-3155-8-34. - DOI - PMC - PubMed
    1. Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PK, Chiu JF, Che CM. Silver nanoparticles: partial oxidation and antibacterial activities. J Biol Inorg Chem. 2007;12:527–534. doi: 10.1007/s00775-007-0208-z. - DOI - PubMed
    1. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol. 2007;73:1712–1720. doi: 10.1128/AEM.02218-06. - DOI - PMC - PubMed

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