Production of Medium Chain Saturated Fatty Acids With Enhanced Antimicrobial Activity From Crude Coconut Fat by Solid State Cultivation of Yarrowia Lipolytica

Food Chem. 2013 Feb 15;136(3-4):1345-9. doi: 10.1016/j.foodchem.2012.09.057. Epub 2012 Sep 28.

Abstract

Fatty acids profiles and antimicrobial activity of crude coconut fat hydrolysates obtained in solid-state cultivation system with a selected yeast strain Yarrowia lipolytica RO13 were performed. A preliminary step regarding extracellular lipase production and solid state enzymatic hydrolysis of crude fat at different water activity and time intervals up to 7 days was also applied. Gas chromatography-mass spectrometry analysis was used for quantification of medium chain saturated fatty acids (MCSFAs) and the results revealed a higher concentration of about 70% lauric acid from total fatty acids. Further, antimicrobial activity of fatty acids against some food-borne pathogens (Salmonella enteritidis, Escherichia coli, Listeria monocytogenes and Bacillus cereus) was evaluated. The minimum inhibitory concentration of the obtained hydrolysates varied from 12.5 to 1.56 ppm, significantly lower than values reported in literature. The results provide substantial evidence for obtaining biopreservative effects by coconut fat enzymatic hydrolysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / analysis
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / pharmacology
  • Bacteria / drug effects
  • Batch Cell Culture Techniques
  • Cocos / chemistry
  • Cocos / metabolism*
  • Culture Media / metabolism
  • Fatty Acids / metabolism*
  • Fatty Acids / pharmacology
  • Gas Chromatography-Mass Spectrometry
  • Microbial Sensitivity Tests
  • Plant Oils / analysis
  • Plant Oils / metabolism*
  • Plant Oils / pharmacology
  • Yarrowia / growth & development
  • Yarrowia / metabolism*

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Fatty Acids
  • Plant Oils