Abstract
The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was obtained from cDNA clones, and its deduced product is a 47,700-kilodalton apoprotein of 424 amino acids. Expression of the HE protein in vitro in the presence of microsomes revealed N-terminal signal peptide cleavage and C-terminal anchorage but not disulfide-linked dimerization. Dimerization was observed only after expression in vivo, during which HE was also transported to the cell surface.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Transport
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Cattle
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Coronaviridae / enzymology
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Coronaviridae / genetics*
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Gene Expression
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Genes, Viral
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Glycoproteins / genetics*
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Glycoproteins / metabolism
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Hemagglutinins, Viral / genetics*
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Hemagglutinins, Viral / metabolism
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Intracellular Membranes / metabolism
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Molecular Sequence Data
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Protein Processing, Post-Translational / genetics
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Viral Fusion Proteins*
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Viral Proteins / genetics*
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Viral Proteins / metabolism
Substances
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Glycoproteins
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Hemagglutinins, Viral
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Viral Fusion Proteins
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Viral Proteins
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hemagglutinin esterase
Associated data
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GENBANK/M30612
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GENBANK/M31052