Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein

J Virol. 1990 Apr;64(4):1834-8. doi: 10.1128/JVI.64.4.1834-1838.1990.

Abstract

The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was obtained from cDNA clones, and its deduced product is a 47,700-kilodalton apoprotein of 424 amino acids. Expression of the HE protein in vitro in the presence of microsomes revealed N-terminal signal peptide cleavage and C-terminal anchorage but not disulfide-linked dimerization. Dimerization was observed only after expression in vivo, during which HE was also transported to the cell surface.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • Cattle
  • Coronaviridae / enzymology
  • Coronaviridae / genetics*
  • Gene Expression
  • Genes, Viral
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism
  • Hemagglutinins, Viral / genetics*
  • Hemagglutinins, Viral / metabolism
  • Intracellular Membranes / metabolism
  • Molecular Sequence Data
  • Protein Processing, Post-Translational / genetics
  • Viral Fusion Proteins*
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Glycoproteins
  • Hemagglutinins, Viral
  • Viral Fusion Proteins
  • Viral Proteins
  • hemagglutinin esterase

Associated data

  • GENBANK/M30612
  • GENBANK/M31052