Oxidized lipids and lysophosphatidylcholine induce the chemotaxis and intracellular calcium influx in natural killer cells

Immunobiology. 2013 Jun;218(6):875-83. doi: 10.1016/j.imbio.2012.10.009. Epub 2012 Oct 26.


We previously reported that human NK cells express G2A and they respond to LPC. Here, we report that oxidized lipids such as 9-R-HODE, 9-S-HODE and 13-R-HODE, as well as LPC induced the in vitro chemotaxis of human NK cells, although with variable efficacies. The chemotactic effects of these lipids were inhibited by prior treatment of NK cells with pertussis toxin (PTX). 9-S-HODE, 9-R-HODE and LPC optimally induced the influx of intracellular Ca(2+) in NK cells. Addition of 9-S-HODE prior to the addition of LPC inhibited more than 50% of the effect of LPC, whereas addition of LPC prior to the addition of 9-S-HODE completely inhibited the effect of the latter lipid. Also, there was a complete reciprocal desensitization among 9-R-HODE and LPC on the influx of intracellular Ca(2+). Further analysis showed that the four lipids did not affect NK cell lysis of tumor target cells. 9-R-HODE but not any other lipid increased the percentages of NK cells producing IFN-γ and is the only lipid that enhanced the release of this cytokine by these cells. In conclusion, we provide novel evidence showing that oxidized lipids and LPC exert important functions for cells of innate immune system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Cells, Cultured
  • Chemotaxis / drug effects*
  • Cytotoxicity, Immunologic / drug effects
  • Cytotoxicity, Immunologic / immunology
  • Dose-Response Relationship, Drug
  • Humans
  • Interferon-gamma / metabolism
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism
  • Ion Transport / drug effects
  • K562 Cells
  • Killer Cells, Natural / drug effects*
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Linoleic Acids / pharmacology
  • Lipids / chemistry
  • Lipids / pharmacology*
  • Lysophosphatidylcholines / pharmacology*
  • Oxidation-Reduction
  • Pertussis Toxin / pharmacology


  • Linoleic Acids
  • Lipids
  • Lysophosphatidylcholines
  • 13-hydroxy-9,11-octadecadienoic acid
  • Interferon-gamma
  • Pertussis Toxin
  • Calcium