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. 2012 Nov 8;13(11):14533-44.
doi: 10.3390/ijms131114533.

Optimization of Pressurized Liquid Extraction of Three Major Acetophenones From Cynanchum Bungei Using a Box-Behnken Design

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Free PMC article

Optimization of Pressurized Liquid Extraction of Three Major Acetophenones From Cynanchum Bungei Using a Box-Behnken Design

Wei Li et al. Int J Mol Sci. .
Free PMC article

Abstract

In this work, pressurized liquid extraction (PLE) of three acetophenones (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for acetophenone extraction.

Figures

Figure 1
Figure 1
Chemical structures of 4-hydroxyacetophenone (1), baishouwubenzophenone (2), and 2,4-dihydroxyacetophenone (3) in C. bungei, and a high-performance liquid chromatogram of an extract of the three acetophenones.
Figure 2
Figure 2
Response surface and contour plots of ethanol concentration and extraction time.
Figure 3
Figure 3
Response surface and contour plots of ethanol concentration and extraction temperature.
Figure 4
Figure 4
Response surface and contour plots of extraction temperature and extraction time.
Figure 5
Figure 5
Extraction yields of acetophenones with different extraction methods.

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