Photo-controllable DNA origami nanostructures assembling into predesigned multiorientational patterns

J Am Chem Soc. 2012 Dec 26;134(51):20645-53. doi: 10.1021/ja307785r. Epub 2012 Dec 14.

Abstract

We demonstrate a novel strategy for constructing multidirectional programmed 2D DNA nanostructures in various unique patterns by introducing photoresponsive oligonucleotides (Azo-ODNs) into hexagonal DNA origami structures. We examined regulation of assembly and disassembly of DNA nanostructures reversibly by different photoirradiation conditions in a programmed manner. Azo-ODNs were incorporated to the hexagonal DNA origami structures, which were then employed as self-assembly units for building up nanosized architectures in regulated arrangements. By adjusting the numbers and the positions of Azo-ODNs in the hexagonal units, the specific nanostructures with face controlling can be achieved, resulting in construction of ring-shaped nanostructures. By combining DNA origami strategy with photoregulating system, remote controlling of assembly and disassembly of DNA nanostructures has been accomplished simply by photo irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • Dimerization
  • Nanostructures / chemistry*
  • Nanostructures / radiation effects
  • Nanostructures / ultrastructure
  • Nanotechnology / methods
  • Nucleic Acid Conformation / radiation effects
  • Oligonucleotides / chemistry*
  • Photochemical Processes

Substances

  • Oligonucleotides
  • DNA