Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells

Mucosal Immunol. 2013 Jul;6(4):838-46. doi: 10.1038/mi.2012.122. Epub 2012 Dec 5.


Although many of the biological features of microfold cells (M cells) have been known for many years, the molecular mechanisms of M-cell development and antigen recognition have remained unclear. Here, we report that Umod is a novel M-cell-specific gene, the translation products of which might contribute to the uptake function of M cells. Transcription factor Spi-B was also specifically expressed in M cells among non-hematopoietic lineages. Spi-B-deficient mice showed reduced expression of most, but not all, other M-cell-specific genes and M-cell surface markers. Whereas uptake of Salmonella Typhimurium via M cells was obviously reduced in Spi-B-deficient mice, the abundance of intratissue cohabiting bacteria was comparable between wild-type and Spi-B-deficient mice. These data indicate that there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Cell Differentiation
  • GPI-Linked Proteins / genetics
  • GPI-Linked Proteins / metabolism
  • Gene Expression Regulation
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / microbiology
  • Mice
  • Mice, Knockout
  • Microvilli / metabolism
  • Organ Specificity / genetics
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology*
  • Peyer's Patches / metabolism*
  • Proto-Oncogene Proteins c-ets / deficiency
  • Proto-Oncogene Proteins c-ets / genetics
  • Proto-Oncogene Proteins c-ets / metabolism*
  • Signal Transduction*
  • Uromodulin / genetics
  • Uromodulin / metabolism


  • Antigens, Surface
  • GPI-Linked Proteins
  • Gp2 protein, mouse
  • Proto-Oncogene Proteins c-ets
  • Spi-B protein, mouse
  • Umod protein, mouse
  • Uromodulin