Molecular cloning and functional characterization of a mouse ccl6 analog gene in the rat

Genet Mol Res. 2012 Nov 12;11(4):3889-98. doi: 10.4238/2012.November.12.6.


Suppression subtractive hybridization was used to analyze differential expression of genes in rat peritoneal macrophages after granulocyte macrophage colony-stimulating factor treatment. We identified and cloned the mouse C10 analog gene in the rat, and named it as ccl6. The full-length cDNA of rat ccl6 was 467 bp, which contains a single-open reading frame and encodes 116 amino acid residues. Compared with other C-C chemokines, the rat ccl6 gene had an unusual four-exon genome structure instead of the typical three exons, it had the highest homology with murine ccl6. The rat ccl6 gene was localized on chromosome 10, where most of the C-C chemokine superfamily members are located. The recombinant rat C-C chemokine ligand 6 (CCL6) protein was expressed by the pGEX4T-1 plasmid in Escherichia coli BL21. The purified recombinant protein had bioactivity similar to that of mouse CCL6, which is a chemoattractant for macrophages and lymphocytes, but not for neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chemokines, CC / chemistry
  • Chemokines, CC / genetics*
  • Chemokines, CC / isolation & purification
  • Chemotaxis / drug effects
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Exons / genetics
  • Hybridization, Genetic / drug effects
  • Introns / genetics
  • Mice
  • Molecular Sequence Data
  • Neutrophils / cytology
  • Neutrophils / drug effects
  • Phylogeny
  • Rats
  • Recombinant Proteins / pharmacology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid*


  • Ccl6 protein, rat
  • Chemokines, CC
  • Recombinant Proteins
  • Ccl6 protein, mouse

Associated data

  • GENBANK/AY620903