Emerging strategies for spatiotemporal control of stem cell fate and morphogenesis

Trends Biotechnol. 2013 Feb;31(2):78-84. doi: 10.1016/j.tibtech.2012.11.001. Epub 2012 Dec 5.

Abstract

Stem cell differentiation is regulated by the complex interplay of multiple parameters, including adhesive intercellular interactions, cytoskeletal and extracellular matrix remodeling, and gradients of agonists and antagonists that individually and collectively vary as a function of spatial locale and temporal stages of development. Current approaches to direct stem cell differentiation focus on systematically understanding the relative influences of microenvironmental perturbations and simultaneously engineering platforms aimed at recapitulating physicochemical aspects of tissue morphogenesis. This review focuses on novel approaches to control the spatiotemporal dynamics of stem cell signaling and morphogenic remodeling to direct the differentiation of stem cells and develop functional tissues for in vitro screening and regenerative medicine technologies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Differentiation*
  • Extracellular Matrix / metabolism
  • Humans
  • Signal Transduction
  • Stem Cells / cytology*
  • Stem Cells / physiology*