Anti-inflammatory effects of carvacrol: evidence for a key role of interleukin-10

Eur J Pharmacol. 2013 Jan 15;699(1-3):112-7. doi: 10.1016/j.ejphar.2012.11.040. Epub 2012 Dec 3.

Abstract

Carvacrol, a phenolic monoterpene, has been reported to possess anti-inflammatory properties. However, the mechanisms involved in its pharmacological properties are currently not well understood. In the present study, the contribution of cytokine modulation to the anti-inflammatory effects of carvacrol was investigated in a classical inflammation model: the complete Freund's adjuvant (CFA)-induced paw inflammation in mice. The paw edema was measured using a plesthismometer. Paw tissue was removed 2h after the inflammatory stimulus to determine the levels of prostaglandin E(2) (PGE(2)) by enzyme immunoassay, the levels of interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) by ELISA or the mRNA expression of cyclooxygenase-2 (COX-2), IL-1β, TNF-α, and IL-10 by real-time PCR. Administration of carvacrol produced anti-inflammatory effects against CFA-induced inflammation in mice. Treatment of mice with carvacrol at 50 and 100mg/kg attenuated the paw edema and reduced the IL-1β and PGE(2), but not TNF-α, local levels. Similarly, carvacrol (100mg/kg) reduced the COX-2 and IL-1β mRNA expression. The levels of IL-10, an anti-inflammatory cytokine, and the IL-10 mRNA expression in the inflamed paw were enhanced by carvacrol. In addition, the treatment with carvacrol did not reduce the CFA-induced paw edema in IL-10 knockout mice. The present results suggest that carvacrol causes anti-inflammatory effects by reducing the production of inflammatory mediators, such as IL-1β and prostanoids, possibly through the induction of IL-10 release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology*
  • Cymenes
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Edema / drug therapy
  • Edema / pathology
  • Gene Expression Regulation / drug effects
  • Inflammation / drug therapy*
  • Inflammation / pathology
  • Inflammation Mediators / metabolism
  • Interleukin-10 / genetics
  • Interleukin-10 / metabolism*
  • Interleukin-1beta / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Monoterpenes / administration & dosage
  • Monoterpenes / pharmacology*
  • Prostaglandins / metabolism
  • RNA, Messenger / metabolism
  • Real-Time Polymerase Chain Reaction

Substances

  • Anti-Inflammatory Agents
  • Cymenes
  • Inflammation Mediators
  • Interleukin-1beta
  • Monoterpenes
  • Prostaglandins
  • RNA, Messenger
  • Interleukin-10
  • carvacrol