Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth

J Virol. 2013 Feb;87(4):2072-80. doi: 10.1128/JVI.02807-12. Epub 2012 Dec 5.

Abstract

N(pro) is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the N(pro) protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N(pro) protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N(pro). Our results suggest that cellular PCBP1 positively modulates CSFV growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Centrifugation
  • Classical Swine Fever Virus / growth & development*
  • Endopeptidases / metabolism*
  • Gene Knockdown Techniques
  • Host-Pathogen Interactions*
  • Humans
  • Immunoprecipitation
  • Microscopy, Confocal
  • Protein Binding
  • Protein Interaction Mapping*
  • RNA, Small Interfering / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Swine
  • Two-Hybrid System Techniques
  • Viral Proteins / metabolism*

Substances

  • RNA, Small Interfering
  • RNA-Binding Proteins
  • Viral Proteins
  • Endopeptidases
  • N(pro) protein, swine fever virus