Toxoflavin lyase enzyme as a marker for selecting potato plant transformants

Biosci Biotechnol Biochem. 2012;76(12):2354-6. doi: 10.1271/bbb.120593. Epub 2012 Dec 7.

Abstract

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genetic Engineering / methods*
  • Genetic Markers / genetics*
  • Lyases / genetics*
  • Lyases / metabolism
  • Pyrimidinones / metabolism
  • Pyrimidinones / pharmacology*
  • Solanum tuberosum / drug effects*
  • Solanum tuberosum / genetics*
  • Transformation, Genetic / drug effects*
  • Triazines / metabolism
  • Triazines / pharmacology*

Substances

  • Genetic Markers
  • Pyrimidinones
  • Triazines
  • toxoflavin
  • Lyases