Generation of integration-free neural progenitor cells from cells in human urine

Nat Methods. 2013 Jan;10(1):84-9. doi: 10.1038/nmeth.2283. Epub 2012 Dec 9.


Human neural stem cells hold great promise for research and therapy in neural disease. We describe the generation of integration-free and expandable human neural progenitor cells (NPCs). We combined an episomal system to deliver reprogramming factors with a chemically defined culture medium to reprogram epithelial-like cells from human urine into NPCs (hUiNPCs). These transgene-free hUiNPCs can self-renew and can differentiate into multiple functional neuronal subtypes and glial cells in vitro. Although functional in vivo analysis is still needed, we report that the cells survive and differentiate upon transplant into newborn rat brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Biomarkers / metabolism
  • Blotting, Western
  • Brain / cytology*
  • Cell Differentiation*
  • Cellular Reprogramming*
  • Epithelial Cells / cytology*
  • Gene Expression Profiling
  • Humans
  • Immunoenzyme Techniques
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / transplantation
  • Neuroglia / cytology
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / genetics
  • Rats
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cell Transplantation
  • Tissue Engineering / methods*
  • Urine / chemistry
  • Urine / cytology*


  • Biomarkers
  • RNA, Messenger

Associated data

  • GEO/GSE36395