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. 2013 Feb;51(2):647-52.
doi: 10.1128/JCM.02927-12. Epub 2012 Dec 5.

Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden

Fabiana C Pimenta  1 , Alexis Roundtree, Ahmet Soysal, Mustafa Bakir, Mignon du Plessis, Nicole Wolter, Anne von Gottberg, Lesley McGee, Maria da Gloria Carvalho, Bernard Beall
Affiliations

Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden

Fabiana C Pimenta et al. J Clin Microbiol. 2013 Feb.

Abstract

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.

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